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Primary Research Data
Below are collections of data
produced by or in collaboration with the Glass Lab at
UCSD.
UCSC Genome Browser visualization files
Each of the files below can be
loaded as custom tracks into the UCSC Genome Browser.
A
couple
of
things
to
note
before
trying
this:
- Pay attention to the GENOME
VERSION. Researchers are constantly trying to improve
reference genomes, and when a new version comes out the absolute
position of everything changes. If you upload these files to the
wrong version, sometimes it will complain because certain chromosomes
or random fragments do not exist. If you get this error make sure
the version is correct. Sometimes it won't complain, which is
worse since all of the peaks etc. will be in the wrong positions.
- DO
NOT
UNZIP these files when uploading them. UCSC takes
zipped files, and if you try to upload an unzipped version you'll find
it's harder to get it to successfully load.
- If you're having trouble loading them, try "right-clicking"
on the links below and "Copy Link Location". You can past this in
to the field when loading custom tracks - this way UCSC will directly
load the file from the biowhat.ucsd.edu server instead of from your
computer.
- Don't like my ugly colors for some of the tracks?
Colors for each experiment were generated randomly, and if you don't
like them, or they are hard to see, or you want to stick to a different
color coding scheme, click on the "manage custom tracks" button and
then click directly on the name of the track. This will give you
access to the configuration data for the track. There will be a
part of the text box with "color=r,g,b" that you can directly modify to
get the color you want.
Notes on Homer UCSC files
Files posted here were created in
BedGraph format. For details on how they were made, click here. Each of these files have
been made such that when zipped they are approximately 50 MB.
This involves smoothing the raw data to a degree so that the file size
doesn't get too big.
All files were updated on 08/31/2010 to correct for a change in the way
UCSC handles the files.
Heinz et al. - PU.1
cistrome in Macrophages and B cells
Data below from:
Macrophage (Thioglycollate Elicited Peritoneal Macrophages)
Naive B cells (Spleen)
Bone Marrow Derived Macrophages
RAW 264.7 (Macrophage-like Cell Line, expressing BirA and
BLRP tagged LXRb)
PUER Cells (PU.1-/- line with PU.1-ER fusion)
pre-proB cells (E2A-/-) [Reconstituted with either wild-type
E2A(E47) or mutant(bHLH)]
pre-proB cells (EBF-/-)
Sullivan et al. - SRF
(Serum Response Factor) cistrome in Macrophages
Data below from:
Macrophage (Thioglycollate Elicited Peritoneal Macrophages)
PUER Cells (PU.1-/- line with PU.1-ER fusion)
Yin et al. - E2A
cistrome in B cells
Data below from:
Lin YC, Jhunjhunwala S, Benner C,
Heinz S, Welinder E, Mansson R, Sigvardsson M, Hagman J, Espinoza CA,
Dutkowski J, Ideker T, Glass CK, Murre C. A
global network of transcription factors, involving E2A, EBF1 and FOXO1,
that orchestrates the B cell fate. Nat Immunol. 2010
Jul;11(7):635-43.
pre-proB Cells (EBF-/-)
proB Cells (RAG1-/-)
pre-proB Cells (E2A-/-)
[Reconstituted with either wild-type
E2A(E47) or mutant(bHLH)]
A12 T Cells (E2A-/-, double
positive) [Reconstituted with
either wild-type E2A(E47) or mutant(bHLH)]
Lymphocyte Inputs
Stender et al. - Estrogen
Receptor (ERa) DNA binding mutant cistrome
Data below from:
MBA-MB-231 (Human Breast Cancer ER-negative Cell Line)
[Stables with either wild-type
ERa or ERa with a mutated DNA-binding domain]
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