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HOMER

Software for motif discovery and next-gen sequencing analysis



Primary Research Data

Below are collections of data produced by or in collaboration with the Glass Lab at UCSD.

UCSC Genome Browser visualization files

Each of the files below can be loaded as custom tracks into the UCSC Genome Browser.  A couple of things to note before trying this:
  1. Pay attention to the GENOME VERSION.  Researchers are constantly trying to improve reference genomes, and when a new version comes out the absolute position of everything changes.  If you upload these files to the wrong version, sometimes it will complain because certain chromosomes or random fragments do not exist.  If you get this error make sure the version is correct.  Sometimes it won't complain, which is worse since all of the peaks etc. will be in the wrong positions.
  2. DO NOT UNZIP these files when uploading them.  UCSC takes zipped files, and if you try to upload an unzipped version you'll find it's harder to get it to successfully load.
  3. If you're having trouble loading them, try "right-clicking" on the links below and "Copy Link Location".  You can past this in to the field when loading custom tracks - this way UCSC will directly load the file from the biowhat.ucsd.edu server instead of from your computer.
  4. Don't like my ugly colors for some of the tracks?  Colors for each experiment were generated randomly, and if you don't like them, or they are hard to see, or you want to stick to a different color coding scheme, click on the "manage custom tracks" button and then click directly on the name of the track.  This will give you access to the configuration data for the track.  There will be a part of the text box with "color=r,g,b" that you can directly modify to get the color you want.

Notes on Homer UCSC files

Files posted here were created in BedGraph format.  For details on how they were made, click here.  Each of these files have been made such that when zipped they are approximately 50 MB.  This involves smoothing the raw data to a degree so that the file size doesn't get too big.

All files were updated on 08/31/2010 to correct for a change in the way UCSC handles the files.


Data from the Glass Lab

Heinz et al. - PU.1 cistrome in Macrophages and B cells

Data below from:

Heinz S, Benner C, Spann N, Bertolino E, Lin YC, Laslo P, Cheng JX, Murre C, Singh H, Glass CK.  Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities. Mol Cell 2010 May 28;38(4):576-589. PMID: 20513432

Link to the raw data: GSE21512

Macrophage (Thioglycollate Elicited Peritoneal Macrophages)

PU.1 [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPa [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me3 [UCSC BedGraph] [Alignment BED] (mm8)
Input [UCSC BedGraph] [Alignment BED] (mm8)

Naive B cells (Spleen)

PU.1 [UCSC BedGraph] [Alignment BED] (mm8)
Oct-2 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me3 [UCSC BedGraph] [Alignment BED] (mm8)
Input [UCSC BedGraph] [Alignment BED] (mm8)

Bone Marrow Derived Macrophages

PU.1 [UCSC BedGraph] [Alignment BED] (mm8)
PU.1 (LXRa/b -/-) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (LXRa/b -/-) [UCSC BedGraph] [Alignment BED] (mm8)

RAW 264.7 (Macrophage-like Cell Line, expressing BirA and BLRP tagged LXRb)

BLRP-LXRb (1h GW3965 [synthetic LXR ligand]) [UCSC BedGraph] [Alignment BED] (mm8)
BirA input (1h GW3965 [synthetic LXR ligand]) [UCSC BedGraph] [Alignment BED] (mm8)

PUER Cells (PU.1-/- line with PU.1-ER fusion)

PU.1 (PU.1-/-) [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb (PU.1-/-) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (PU.1-/-) [UCSC BedGraph] [Alignment BED] (mm8)

PU.1 (0h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb (0h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (0h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
MNase-Seq (mononucleosomes) (0h tamoxifen) [No UCSC File] [Alignment BED] (mm8)

PU.1 (1h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb (1h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (1h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
MNase-Seq (mononucleosomes) (1h tamoxifen) [No UCSC File] [Alignment BED] (mm8)

PU.1 (6h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb (6h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)

PU.1 (24h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb (24h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (24h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)

PU.1 (48h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)
C/EBPb (48h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8)

pre-proB cells (E2A-/-) [Reconstituted with either wild-type E2A(E47) or mutant(bHLH)]

PU.1 [UCSC BedGraph] [Alignment BED] (mm8)
PU.1 (E47ER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
PU.1 (bHLHER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)

pre-proB cells (EBF-/-)

PU.1 [UCSC BedGraph] [Alignment BED] (mm8)

proB cells (RAG1-/-)



Sullivan et al. - SRF (Serum Response Factor) cistrome in Macrophages

Data below from:

Sullivan AL, Benner C, Heinz S, Huang W, Xie L, Miano JM, Glass CK. SRF utilizes distinct promoter and enhancer-based mechanisms to regulate cytoskeletal gene expression in macrophages. Mol Cell Biol. 2010 Dec 6. PMID: 21135125

Macrophage (Thioglycollate Elicited Peritoneal Macrophages)

SRF [UCSC BedGraph] [Alignment BED] (mm8) [FASTQ]
SRF in SRF-/- mice [UCSC BedGraph] [Alignment BED] (mm8) [FASTQ][FASTQ]
PU.1 in SRF-/- mice [UCSC BedGraph] [Alignment BED] (mm8) (for wild type PU.1 see above) [FASTQ]

PUER Cells (PU.1-/- line with PU.1-ER fusion)

SRF (PU.1-/-) [UCSC BedGraph] [Alignment BED] (mm8) [FASTQ]
SRF (0h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8) [FASTQ]
SRF (24h tamoxifen) [UCSC BedGraph] [Alignment BED] (mm8) (for wild type PU.1 see above) [FASTQ]


Data from the Murre Lab

Yin et al. - E2A cistrome in B cells

Data below from:

Lin YC, Jhunjhunwala S, Benner C, Heinz S, Welinder E, Mansson R, Sigvardsson M, Hagman J, Espinoza CA, Dutkowski J, Ideker T, Glass CK, Murre C.  A global network of transcription factors, involving E2A, EBF1 and FOXO1, that orchestrates the B cell fate. Nat Immunol. 2010 Jul;11(7):635-43.

Link to the Raw Data: GSE21978

pre-proB Cells (EBF-/-)

E2A [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me2 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me3 [UCSC BedGraph] [Alignment BED] (mm8)
H3K27me3 [UCSC BedGraph] [Alignment BED] (mm8)
H3K9ac/H3K14ac [UCSC BedGraph] [Alignment BED] (mm8)

proB Cells (RAG1-/-)

E2A [UCSC BedGraph] [Alignment BED] (mm8)
EBF [UCSC BedGraph] [Alignment BED] (mm8)
CTCF [UCSC BedGraph] [Alignment BED] (mm8)
Foxo1 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me2 [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me3 [UCSC BedGraph] [Alignment BED] (mm8)
H3K27me3 [UCSC BedGraph] [Alignment BED] (mm8)
H3K9ac/H3K14ac [UCSC BedGraph] [Alignment BED] (mm8)

pre-proB Cells (E2A-/-) [Reconstituted with either wild-type E2A(E47) or mutant(bHLH)]

E2A (E47ER, 1h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
E2A (E47ER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (E47ER, 1h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (E47ER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (bHLHER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)

A12 T Cells (E2A-/-, double positive) [Reconstituted with either wild-type E2A(E47) or mutant(bHLH)]

E2A (E47ER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (E47ER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)
H3K4me1 (bHLHER, 6h after tamoxifen treatment) [UCSC BedGraph] [Alignment BED] (mm8)

Lymphocyte Inputs

Naive B cell Input [UCSC BedGraph] [Alignment BED] (mm8)
Lymphoid Cells [UCSC BedGraph] [Alignment BED] (mm8)


Data from the Katzenellenbogen Lab

Stender et al. - Estrogen Receptor (ERa) DNA binding mutant cistrome

Data below from:

Stender JD, Kim K, Charn TH, Komm B, Chang KC, Kraus WL, Benner C, Glass CK, Katzenellenbogen BS. Genome-Wide Analysis of Estrogen Receptor-{alpha} DNA Binding and Tethering Mechanisms Identifies Runx1 as a Novel Tethering Factor in Receptor-Mediated Transcriptional Activation. Mol Cell Biol. 2010 Jun 14.

Link to the raw data: GSE22610

MBA-MB-231 (Human Breast Cancer ER-negative Cell Line) [Stables with either wild-type ERa or ERa with a mutated DNA-binding domain]

ERa (Estradiol) [UCSC BedGraph] [Alignment BED] (hg18)
ERa-mutant (Estradiol) [UCSC BedGraph] [Alignment BED] (hg18)










Can't figure something out? Questions, comments, concerns, or other feedback:
cbenner@ucsd.edu